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based approach to model patterns Aztreonam chemoresistance and tumor recurrence using ovarian cancer stem cell spheroids. Clin. Cancer Res. 23, 6934–6945.
Weerapana, E., Speers, A.E., and Cravatt, B.F. (2007). Tandem orthogonal proteolysis-activity-based protein profiling (TOP-ABPP)—a general method for mapping sites of probe modification in proteomes. Nat. Protoc 2, 1414– 1425.
KEY RESOURCES TABLE
REAGENT or RESOURCE
Bacterial and Virus Strains
Subcloning Efficiency DH5a Competent Cells
Patient-derived xenografts PDXs
Charles Landen lab
Patient tumor samples
Chemicals, Peptides, and Recombinant Proteins
Pan-Caspase inhibitor Z-VAD-FMK
Caspase 3 Inhibitor Z-DEVD-FMK
Critical Commercial Assays
Fluo Calcium Indicator CM-H2DCF
SuperScript III First strand synthesis SuperMix
CCLE cell line analysis-ALDH1A expression
673A PubChem search
CM10 PubChem search
Experimental Models: Cell Lines
Experimental Models: Organisms/Strains
The Jackson labs
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REAGENT or RESOURCE
TransOmics clone BC008392
TransOmics Clone BC096
CONTACT FOR REAGENT AND RESOURCE SHARING
Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact, Ron Buckanovich ([email protected]).
EXPERIMENTAL MODEL AND SUBJECT DETAIL
2) Primary samples: primary ovarian cancer tumors were obtained after surgery and dissociated to single cell suspension. Single cell suspension was used for spheroid studies.
3) LSL-K-ras G12D/+ Pten loxP/loxP was established by Dr. Romero, OBGYN department, University of Chicago
4) PDX model of ovarian cancer was established by Dr. Charles Landen, OBGYN department, University of Virginia and Dr. Buck-anovich University of Michigan.
5) NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice from Jackson labs #00557 were used for all subcutaneous and IP injections
Cell culture and reagents
PEO1, PEO4, OvCar8, and Kuramochi were used in experiments that assessed 673A effect on CD133 cells due to very high CD133+ expression. A2780, Ovsaho, and OVCAR5 cells were used in experiments which required very distinctive CD133+ population. SKoV3 were used in experiments that required very bright ALDH+ population. OvCar5 and OvCar8 used due to their high ALDH1A3 expression.